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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: The Organophosphate Degradation ( opd ) Island-borne Esterase-induced Metabolic Diversion in Escherichia coli and Its Influence on p -Nitrophenol Degradation
doi: 10.1074/jbc.M115.661249
Figure Lengend Snippet: PNP supported growth of E. coli MG1655 (pSDP10). A indicates growth of MG1655 (pSDP10), MG1655 (pMMB206), and MG1655 cells in PNP-containing minimal medium. Closed (growth) and open (PNP) circles represent growth and PNP concentration in culture medium containing MG1655 (pSDP10). Similar parameters are indicated with closed (growth) and open (PNP) squares for MG1655 (pMMB206) and closed (growth) and open (PNP) rhombuses for MG1655 cultures. Proteins extracted from MG1655 (pSDP10) cells grown in the presence of normal and 14C-labeled PNP were analyzed by SDS-PAGE, and incorporation of 14C into proteins is shown in the corresponding autoradiogram (B). RNA extracted from similarly grown cultures and the corresponding autoradiogram are shown in C. The intense sharp signal shown by the arrow indicates incorporation of 14C into 5S rRNA. D indicates a decrease in concentration of PNP and concomitant release of stoichiometric amounts of nitrite in resting cells of E. coli (pSDP10). HPLC profiles showing the time-dependent decrease of PNP in the culture medium and the appearance of nitrocatechol (4.5 min) and benzenetriol (8.3 min) are shown in E and F. AU, absorbance units; ND, not detected.
Article Snippet: Probe Design The
Techniques: Concentration Assay, Labeling, SDS Page
Journal: The Journal of Biological Chemistry
Article Title: The Organophosphate Degradation ( opd ) Island-borne Esterase-induced Metabolic Diversion in Escherichia coli and Its Influence on p -Nitrophenol Degradation
doi: 10.1074/jbc.M115.661249
Figure Lengend Snippet: The Orf306-dependent induction of the hca and mhp operons. A, heat map showing expression of the hca and mhp operons at 0, 1.5, and 3.0 h after induction of orf306. The portion of the two-dimensional gel showing Orf306-specific induction of HcaR and MhpA proteins is shown in B. The quantification (qPCR) of mhpA-, mhpR-, hcaE-, and hcaR-specific transcripts under similar growth conditions is shown in C with the error bars representing the S.D. Growth of the MG1655 (pDS10) (●) and hcaE (■), mhpA (▴), and mhpR (♦) mutants in PNP-containing minimal medium is shown in D. E represents agarose gel pictures indicating deletion of hcaE, mhpA, and mhpR genes. The PNP-dependent growth of MG1655 (pDS10) (●) and paaA (■) and maoA (▴) mutants (F) and an agarose gel showing the deletion of the paaA and maoA genes (G) are shown.
Article Snippet: Probe Design The
Techniques: Expressing, Two-Dimensional Gel Electrophoresis, Agarose Gel Electrophoresis
Journal: The Journal of Biological Chemistry
Article Title: The Organophosphate Degradation ( opd ) Island-borne Esterase-induced Metabolic Diversion in Escherichia coli and Its Influence on p -Nitrophenol Degradation
doi: 10.1074/jbc.M115.661249
Figure Lengend Snippet: Orf306-induced metabolic diversion in MG1655 (pSDP10). Heat maps represent differential expression of genes encoding glycolysis, glyoxylate, and TCA cycle enzymes at 0, 1.5, and 3.0 h. The green arrows represent down-regulated pathways. The up-regulated pathways are shown with red arrows. Up-regulation of the phenylpropionate pathway and down-regulation of glycolysis are shown with dotted green and red arrows, respectively. Quantitative PCR results showing either an Orf306-dependent decrease (pfkA, eno, and acnA) or increase (glcB, glcC, and prpB) in the concentration of specific mRNAs are inserted at places showing the corresponding enzyme reactions with the error bars representing the S.D. The two-dimensional gel portions indicate Orf306-dependent induction of MhpA, HcaR, GlcC, PrpB, and SdhA. Eno, enolase; PEP, phosphoenolpyruvate.
Article Snippet: Probe Design The
Techniques: Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Two-Dimensional Gel Electrophoresis
Journal: The Journal of Biological Chemistry
Article Title: The Organophosphate Degradation ( opd ) Island-borne Esterase-induced Metabolic Diversion in Escherichia coli and Its Influence on p -Nitrophenol Degradation
doi: 10.1074/jbc.M115.661249
Figure Lengend Snippet: Propionate-dependent induction of hcaR and mhpR genes. The lacZ-negative strains of MG1655 containing promoter test vector pMP220 and hcaR-, mhpR-, and mhpA-lacZ fusions were grown either on a propionate + X-gal (A) or a glucose + X-gal plate (B). The quantification of promoter activity for propionate- and glucose-grown cultures is shown in C and D, respectively, with the error bars representing the S.D. The Orf306-dependent induction of the hcaR, mhpR, and mhpA genes in MG1655 (pSDP10) cells is shown in E and F, respectively.
Article Snippet: Probe Design The
Techniques: Plasmid Preparation, Activity Assay